The full curated list of my publications can be found on ResearchIdor ORCID. My name is rather common, therefore, if you wish to see my record on PubMed or Google Scholar, you have to click on the links provided. My research output span from theoretical work in the area of information theory, engineering, biophysics to biological applications. Within my limited time, I am trying to describe in this website my research output, but in this page you can find my research output in terms of publications, my relative contribution as an author and bibliometrics. It is in the spirit of the San Francisco Declaration on Research Assessment that I have designed this website, but nevertheless for full disclosure, I here provide this list and statistics.

You might notice four main distinct phases in my research output:

  1. My doctoral studies, during which I had a rather eclectic research output. I was a prolific student publishing work on theory, technology, biotechnology and biological applications. My focus was fluorescence lifetime imaging microscopy (FLIM) and neurodegeneration.
  2. My early work in Cambridge (UK), when we successfully applied a number of microscopy techniques to the study of red blood cells infected by Plasmodium falciparum, the causative pathogen of malaria.
  3. My stint as an EPSRC LSI fellow during which I focused on the engineering of novel multi-parametric imaging techniques for multiplexed biochemical imaging and, at the same time I refocused my work on what is now my only subject of investigation, cancer research.
  4. My reduced output after 2012, during which I have been entirely focusing on deploying these methodologies, more recently with a help of a great team of people, to the study of cellular decisions in cancer. I presented this work at conferences already, and – in part – also in this website, but it will be soon shared in journals and repositories.

June 2018: ISI – citations (1046) and h-index (21)

June 2018: Google Scholar – citations (1615) and h-index (26)


Legend: Shared first or senior authorship; * Papers where I am correspondent author

Under review:

  • Maximilian W Fries, Kalina T Haas, Suzan Ber, John Saganty, Emma K Richardson, Ashok R Venkitaraman, and Alessandro Esposito,*, “Multiplexed biochemical imaging reveals caspase activation patterns underlying single cell fate”, online at bioaRxiv [BIORXIV/2018/427237] while under review
  • Alessandro Esposito* and Ashok R. Venkitaraman. “Enhancing biochemical resolution by hyper-dimensional imaging microscopy”, online at bioaRxiv [BIORXIV/2018/431452] while under review
  • Alessandro Esposito. “Cooperation of partially-transformed clones: the invisible force behind the early stages of carcinogenesis”,  online at bioaRxiv [BIORXIV/2018/431478
  • Callum J. Campbell, Ashok R. Venkitaraman and Alessandro Esposito*, “Checkpoint non-fidelity induces a complex landscape of lineage fitness after DNA damage”, online at bioaRxiv [BIORXIV/2018/431486

  • Siddharth De, Callum J Campbell, Ashok R Venkitaraman, and Alessandro Esposito,*, “Pulsatile MAPK signalling modulates p53 activity to control cell fate decisions at the G2 checkpoint for DNA damage”, in review


  • Kalina T. Haas, MiYoung Lee, Alessandro Esposito† and Ashok R. Venkitaraman† (2018), “Single-molecule localization microscopy reveals molecular transactions during RAD51 filament assembly at cellular DNA damage sites”, Nucleic Acids Research 46(5):2398–2416
  • Popleteeva A, Stoppa D, Pancheri L, Gasparini L, Kaminski CF, Cassidy LD, Venkitaraman AR, Esposito A* (2015), “Fast and simple spectral FLIM for biochemical and medical imaging”, Optics Express 23(18):23511-23525
  • Liang H, Esposito A, De S, Ber S, Collin P, Surana U, Venkitaraman AR, “Homeostatic control of the G2 checkpoint via polo-like kinase 1 engenders non-genetic heterogeneity in its fidelity and timing”, Nat Comms. 5, 4048
  • Esposito A*, Popleteeva M, Venkitaraman AR, “Maximizing the biochemical resolving power in fluorescence microscopy”, PLOS ONE, 8(10):e77392
  • Kiwanuka S, Laurila T, Jonathan F, Esposito A, Blomberg von der Geest K, Pancheri L, Stoppa D, Kaminski C, “Development of broadband cavity ring-down spectroscopy for biomedical diagnostics of liquid analytes”, Analytical Chemistry, 84 (13):5489–5493
  • Esposito A*, “Beyond range: innovating fluorescence microscopy”, Remote Sens. 4(1):111-119
  • Fereidouni F, Esposito A, Blab GA, Gerritsen HC, “A modified phasor approach for analyzing time-gated fluorescence lifetime images”, J. Microsc. 244(3):248-258
  • Esposito A*, Bader AN, Schlachter SC, van den Heuvel DJ, Kaminski Schierle GS, Venkitaraman AR, Kaminski CF, Gerritsen HC, “Design and application of a confocal microscope for spectrally resolved anisotropy imaging”, Opt. Express, 19(3):2546–2555
  • Kaminski Schierle GS, Bertoncini CW, Chan FTS, van der Goot AT, Schwedler S, Skepper J, Schlachter S, van Ham T, Esposito A, Kumita JR, Nollen EAA, Dobson CM, Kaminski CF, “A FRET sensor for non-invasive imaging of amyloid formation in vivo“, ChemPhysChem, 12(3):673-680
  • Mauritz JMA, Seear R, Esposito A, Kaminski CF, Skepper J, Warley A, Lew VL, Tiffert T, “X-ray Microanalysis Investigation of the Stage-Related Changes in Na, K and Hemoglobin Concentration in Plasmodium Falciparum -Infected Red Blood Cells”, Biophys. J., 100(6):1438-1445
  • Jeyasekharan AD, Ayoub N, Mahen R, Ries J, Esposito A, Rajendra E, Hattori H, Kulkarni RP, Venkitaraman AR, “DNA damage regulates the mobility of Brca2 within the nucleoplasm of living cells”, Proc. Natl. Acad. Sci. USA, 107(50): 21937–21942.
  • Swietach P, Tiffert T, Mauritz JM, Seear R, Esposito A, Kaminski C, Lew VL, Vaughan-Jones RD, “Hydrogen ion dynamics in human red blood cells”, J Physiol., 588(24):4995-5014
  • Mauritz J, Esposito A, Tiffert T, Skepper JN, Warley A, Yoon YZ, Cicuta P, Lew VL, Guck JR, Kaminski CF, “Biophotonic techniques for the study of malaria-infected red blood cells”, Med Biol Eng Comput., 48(10):1055-63
  • Mauritz J, Tiffert T, Seear R, Lautenschlaeger F, Esposito A, Lew V, Guck J, Kaminski CF, “Detection of Plasmodium falciparum -infected red blood cells by optical stretching”, J. Biomed. Opt., 15(3):030517
  • Esposito A*, Choimet JB, Skepper JN, Mauritz JMA, Lew VL, Kaminski CF, Tiffert T, “Quantitative imaging of human red blood cells infected with Plasmodium falciparum“, Biophys. J., 99(3):953-960
  • Cheung W, Gill M, Esposito A, Kaminski CF, Courousse N, Chwetzoff S, Trugnan G, Keshavan N, Lever A and Desselberger U , “Rotaviruses associate with cellular lipid droplet components to replicate in viroplasms, and compounds disrupting or blocking lipid droplets inhibit viroplasm formation and viral replication”, J. Virol. 84(13): 6782-6798
  • Schlachter S, Schwedler S, Esposito A, Moggridge GD, Kaminski GS and Kaminski CF, “A method to unmix multiple fluorophores in microscopy images with minimal a priori information”, Opt. Express, 17(25):22747-22760
  • Cueff A, Dyrda A, Bouyer G, Egée S, Seers R, Esposito A, Tiffert T, Lew VL and Thomas SLY , “Effects of elevated intracellular calcium on the osmotic fragility of human red blood cells”, Cell Calcium, 47(1):29-36
  • Wagner OI, Esposito A, Shen K, Wenzel D, Koehler B, Wouters FS and Klopfenstein DR , “Synaptic scaffolding protein SYD-2 clusters and activates kinesin-3 UNC-104 in C. elegans“, Proc. Natl. Acad. Sci. USA, 106(46):19605-10
  • van Ham TJ, Esposito A, Kumita JR, Hsu SD, Kaminski-Schierle GS, Kaminski CF, Dobson CM, Nollen EAA and Bertoncini CW , “Towards multiparametric fluorescent imaging of amyloid formation: Studies on a YFP model of alpha-synuclein aggregation”, J Mol Biol 395(3):627-642
  • Mauritz J, Esposito A*, Ginsburg H, Kaminski CF, Tiffert T and Lew VL, “The Homeostasis of Plasmodium falciparum Infected Red Blood Cells”, PLoS Comp Biol 5(4):e1000339.doi:10.1371/journal.pcbi.1000339
  • Schlachter S, Elder AD, Esposito A, Kaminski GS, Frank GH, van Geest LK, and Kaminski CF, “mhFLIM: Resolution of heterogeneous fluorescence decays in widefield lifetime microscopy”, Opt Express 17(3):1557-1570
  • Elder AD, Domin A, Kaminski Schierle GS, Lindon C, Pine J, Esposito A, and Kaminski CF, “A quantitative protocol for dynamic measurements of protein interactions by FRET-sensitized fluorescence emission”. Journal of the Royal Society Interface. J R Soc Interface 6(S1):S59-S81
  • Babuke T, Ruonala M, Meister M, Amaddii M, Genzler C, Esposito A, Tikkanen R, “Hetero-oligomerization of reggie-1/flotillin-2 and reggie-2/flotillin-1 is required for their endocytosis”. Cell Signal, 21(8):1287-97
  • Papusheva E, Mello de Queiroz F, Dalous J, Han Y, Esposito A, Jares-Erijman E, Jovin TM, and Bunt G, “Dynamic conformational changes in the FERM domain of FAK are involved in focal adhesion behavior during cell spreading and motility”. J Cell Sci 122, 656-666
  • Esposito A* , Tiffert T, Mauritz J, Schlachter S, Bannister LH, Kaminski CF, Lew VL (2008), “FRET Imaging of Haemoglobin Concentration in Plasmodium Falciparum Infected Erythrocytes”, PLoS ONE, 3(11): e3780. doi:10.1371/journal.pone.0003780
  • Esposito A* , Gralle M, Dani MAC, Lange D, Wouters FS (2008), “pHlameleons: a family of FRET-based protein sensors for quantitative pH imaging”, Biochemistry, Biochemistry, 47(49):13115–13126
  • Wouters FS and Esposito A (2008), “Quantitative analysis of fluorescence lifetime imaging made easy”. HFSP Journal 2(1):7-11 (commentary paper)
  • Esposito A*, Gerritsen HC, Wouters FS (2007), “Optimizing frequency-domain fluorescence lifetime sensing for high throughput applications: photon-economy and acquisition speed”. J. Opt. Soc. Am. A 24(10):3261-3273
  • Esposito A*, Dohm CP, Bähr M and Wouters FS (2007), “Unsupervised Fluorescence Lifetime Imaging Microscopy for High-Content and High-Throughput Screening”. Mol. Cell. Proteomics 6:1446-1454
  • Esposito A*†, Dohm CP†, Kermer P, Bähr M, Wouters FS (2007), “α-synuclein and its disease-related mutants interact differentially with the microtubule protein tau and associate with the actin cytoskeleton”. Neurobiol. Dis. 26(3): 521-531
  • Dohm CP, Siedenberg S, Liman J, Esposito A, Wouters FS, Reed JC, Bähr M, and Kermer P (2006), “Bax inhibitor-1 protects neurons from oxygen-glucose deprivation”. J. Mol Neurosci. 29(1):1-8
  • Esposito A*, Gerritsen HC, Oggier T, Lustenberger F, Wouters FS (2006), “Innovating lifetime microscopy: a compact and simple tool for the life sciences, screening and diagnostics”. J. Biomed. Opt. 11(3):34016-34024
  • Esposito A*, Oggier T, Gerritsen HC, Lustenberger F, Wouters FS (2005), “All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing”. Opt. Express 13(24):9812-9821
  • Esposito A*, Gerritsen HC, Wouters FS (2005), “Fluorescence lifetime heterogeneity resolution in the frequency domain by lifetime moments analysis”. Biophys. J. 89(6):1-14
  • Esposito A*, Cupello A, Pellistri F, Marchetti C, Robello M (2005), “Two-photon analysis of lead accumulation in cerebellar granule cells”. Neurochem. Res. 30(8):949-54
  • Cupello A, Esposito A, Marchetti C, Pellistri F, Robello M (2005), “Calcium accumulation in neurites and cell bodies of rat cerebellar granule cells in culture: effects on GABA(A) receptor function”. Amino Acids 28(2):177-182
  • Pellistri F, Cupello A, Esposito A, Marchetti C, Robello M (2004), “Two-photon imaging of calcium accumulation in rat cerebellar granule cells.”. Neuroreport 15(1):83-87
  • Esposito A, Federici F, Usai C, Cannone F, Chirico G, Collini M, Diaspro A (2004), “Notes On Theory And Experimental Conditions Behind Two-Photon Excitation Microscopy”. Microsc. Res. Tech.63(1):12-17

Book Chapters

  • Esposito A*, Schlachter S, Kaminski Schierle GS, Elder AD, Diaspro A, Wouters FS, Kaminski CF, Iliev AI, “Quantitative fluorescence microscopy techniques” in Cytoskeleton Methods and Protocols Ed.: Ray H. Gavin, Humana Press Series Methods in Molecular Biology). In press
  • Gerritsen HC, Agronskaia S, Bader A, Esposito A (2008), “Time Domain FLIM: theory and Instrumentation” in FRET & FLIM Imaging Techniques, Gadella TWG ed.; Series Laboratory techniques in biochemistry & molecular biology, van der Vliet PC and Pillai S eds.
  • Esposito A, Gerritsen HC, Wouters FS (2008), “Fluorescence lifetime imaging microscopy: quality assessment and standards”, in Standardization in Fluorometry: State-of-the Art and Future Challenges Ed.: Resch-Genger U (Springer Series Methods and Applications of Fluorescence, Ed.: Wolfbeis O)
  • Esposito A, Wouters FS (2004), “Fluorescence Lifetime Imaging Microscopy”, in Current Protocols in Cell Biology. Eds.:Bonifacino JS, Dasso M, Harford JB, Lippincott-Schwartz J, and Yamada KM, John Wiley & Sons, New York, NY.

Patent applications

  • 2007, Esposito A, Wouters FS, Lustenberger F, Oggier T, “Apparatus and method for fluorescence lifetime imaging” EP1746410 and US 2007/0018116 A1 (bought by Carl Zeiss GmbH)


  • PhD thesis: “Molecular and Cellular Quantitative Microscopy”, Cuvillier Verlag (Goettingen, Germany) ISBN-10: 3865378293; Prof. H.Gerritsen (supervisor) and Prof. F.Wouters (co-supervisor)
  • Degree thesis: “Two-photon analysis of intracellular divalent ions in rat cerebellar granules culture” Prof. M.Robello (supervisor) and Dr. C.Marchetti (co-supervisor)